rhinoceros can attack different parts of the coconut trees.

Severe attack rates by this pest affects tree damage ranging from 50 to 100% (Manjeri et al. (Coleoptera: Scarabaeidae) is one of the important pests on coconut and oil palm that causes production losses and young plant mortality reaching 60% and 25%, respectively (Fauzana et al., 2018). majus isolates and CRB-isolated strains suggests a possible relationship between pathogenicity and host specificity with phylogenetic status or DNA sequence polymorphisms.

majus isolates exhibited high pathogenicity against O. The local species of Metarhizium have been isolated from infected CRB larvae, and M. This result indicated a possible relationship between phylogenetic status or DNA sequence polymorphisms with Metarhizium pathogenicity and host specificity. rhinoceros larval cadaver with a faster lethal time (for 50% mortality). In addition, the isolate MaMa had the highest virulence against O. Neighbor-joining analysis showed a close resemblance between the isolate MaMa and the strain ARSEF 1946, which was isolated from the CRB. rhinoceros larvae than other Metarhizium species. majus and MALC had higher pathogenicity against O. The present investigation reported that isolates MaLe, MaMa, MaWa, and MaSi produced higher mortality values than other isolates, supporting that M. anisopliae) in the MGT clade, known as MALC ( M. Interestingly, the isolate MaSi produced conidial lengths well above the range of conidial sizes recorded and placed these isolates ( M. Identified Metarhizium species exhibited varied sizes of conidia, but fell within the size ranges reported in previous studies. Thirteen isolates were obtained which were divided into 4 clades based on phylogenetic analysis by the ITS rDNA region, namely M. This study aimed to identify Metarhizium species isolated from infected CRB larvae with high pathogenicity to host pests in East Java, Indonesia.

The identification of local EPF species is a strategic approach to develop potential biopesticides with standard properties including host specificity, climate suitability, and significantly suppressing the target pest population. Several studies have proven that the native fungal isolates have strong pathogenic activity than EPF introduced from other regions. Application of this fungus as a biopesticide is influenced by regional environmental conditions that affect the pathogenic activity against the targeted pest. Metarhizium is one of the entomopathogenic fungi (EPF) that has been widely reported as a useful agent for controlling the coconut rhinoceros beetle (CRB), Oryctes rhinoceros L.

Coconut rhinoceros beetle indonesia